The Multi-Species Creatinine Detection Kit for Plasma and Serum is designed to quantitatively measure creatinine present in serum and plasma samples. Strip well format. Reagents for up to 96 tests. A creatinine standard, calibrated to a NIST creatinine standard, is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or samples are pipetted into a clear microtiter plate. An assay diluent is added to all standards, controls and samples. The color generating reaction is initiated with the Creatinine Reagent, which is pipetted into each well. The assay utilizes a kinetic absorbance method to overcome interference by colored compounds in serum. The absorbance of the colored product is read after 1 minute in a microtiter plate reader capable of measuring 490nm wavelength. At 30 minutes the optical density is read again. The concentration of creatinine is calculated using the delta of the optical density readings at 30 and 1 minute compared to the curve generated from the standards. The Jaffe reaction used in this kit has been modified to read creatinine levels in serum. More Details: Detection Target: Creatine Reactivity: Mouse, Human Range: 0.78 to 1.45 Sensitivity: 0.081 mg/dL At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results. Please note ¢ this product replaces our old product number IRAAKT2508 or IRAAKT2548 (same product, new number).