This Mouse Tumor Necrosis Factor Receptor Superfamily Member 18 (CD357) ELISA Kit from Innovative Research is intended for quantitative detection of mouse TNFRSF18 in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). Strip well format. Reagents for up to 96 tests.
This mouse TNFRSF18 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for TNFRSF18 has been precoated onto 96-well plates. Standards(NSO, S22-H153) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFRSF18 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse TNFRSF18 amount of sample captured in plate.
- Detection Target: Tumor Necrosis Factor Receptor Superfamily Member 18 (CD357)
- Uniprot ID: )
- Reactivity: Mouse
- Cross-Reactivity: There is no detectable cross-reactivity with other relevant proteins.
- Range: 62.5pg/ml-4000pg/ml
- Sensitivity: <10pg/ml
- Storage Conditions: Store at 4?C for 6 months, at -20?C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice)
Additional Information: The capture antibody is a monoclonal antibody from rat, the detection antibody is a biotinylated polyclonal antibody from goat. Expression system for standard: Tumor necrosis factor receptor superfamily member 18(TNFRSF18), also called GITR or AITR is a protein that in humans is encoded by the TNFRSF18 gene. This gene is mapped to 1p36.33. This gene encodes a member of the TNF-receptor superfamily. The encoded receptor has been shown to have increased expression upon T-cell activation, and it is thought to play a key role in dominant immunological self-tolerance maintained by CD25( )CD4( ) regulatory T cells. Knockout studies in mice also suggest the role of this receptor is in the regulation of CD3-driven T-cell activation and programmed cell death. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.; Immunogen sequence: 369
