Mouse Interleukin 5 Receptor Alpha ELISA Kit from Innovative Research is intended for the quantitative determination of Mouse Interleukin 5 Receptor Alpha in biofluid samples, such as serum, plasma and other biological fluids. This is a sandwich ELISA in strip well format with reagents for up to 96 tests . IL5Ra present in samples reacts with an anti-Mouse IL5Ra capture antibody coated and dried on a microtiter plate. After appropriate washing steps, an anti-IL5Ra detection antibody conjugated to biotin binds to the captured analyte. Excess antibody is washed away and bound antibody is then reacted with avidin conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. Color development is directly proportional to the concentration of active IL5Ra in the samples. A standard calibration curve is prepared along with the samples to be measured using dilutions of Mouse IL5Ra. Detection Target: Mouse Interleukin 5 Receptor Alpha Reactivity: Mouse Range: 62.5-4000pg/mL Sensitivity: 28.5pg/mL Storage Conditions: Store all components at 4┬░C This Mouse IL5Ra activity assay is for the quantitative determination of Interleukin 5 Receptor Alpha in samples including serum, plasma and other biological fluids. For research use only. Strip well format. Reagents for up to 96 tests. For research use only. At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results.