Mouse ATPase H /K Exchanging Alpha Polypeptide ELISA Kit from Innovative Research is intended for the quantitative determination of Mouse ATPase, H /K Exchanging Alpha Polypeptide in biofluid samples, such as tissue homogenates and other biological fluids. This is a sandwich ELISA in strip well format with reagents for up to 96 tests . ATP4a present in samples reacts with an anti-Mouse ATP4a capture antibody coated and dried on a microtiter plate. After appropriate washing steps, an anti-ATP4a detection antibody conjugated to biotin binds to the captured analyte. Excess antibody is washed away and bound antibody is then reacted with avidin conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. Color development is directly proportional to the concentration of active ATP4a in the samples. A standard calibration curve is prepared along with the samples to be measured using dilutions of Mouse ATP4a. Detection Target: Mouse ATPase, H /K Exchanging Alpha Polypeptide Reactivity: Mouse Range: 0.156-10ng/mL Sensitivity: 0.055ng/mL Storage Conditions: Store all components at 4┬░C This Mouse ATP4a activity assay is for the quantitative determination of ATPase, H /K Exchanging Alpha Polypeptide in samples including tissue homogenates and other biological fluids. For research use only. Strip well format. Reagents for up to 96 tests. For research use only. At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results.